High Performance Liquid Chromatography - Mass Spectrometry and Liquid Chromatography (HPLC / MS)

Definition - What does High Performance Liquid Chromatography - Mass Spectrometry and Liquid Chromatography (HPLC / MS) mean?

High Performance Liquid Chromatography - Mass Spectrometry (HPLC-MS), or alternatively Liquid Chromatography - Mass Spectrometry (LC-MS), is a chemistry technique used to analyze substances. For example, urine samples to test for drug use. This technique combines the mass analysis that mass spectrometry (MS) is capable of, with the capability to perform physical separation of the liquid chromatography (HPLC).

WorkplaceTesting explains High Performance Liquid Chromatography - Mass Spectrometry and Liquid Chromatography (HPLC / MS)

High Performance Liquid Chromatography - Mass Spectrometry (HPLC-MS) is a high performance liquid chromatography system with a mass spectrometry detector. Liquid Chromatography (LC) is the technique that laboratories use in order to separate samples into individual parts. The separation happens based on the interactions of the stationary and mobile phases with the sample. High Performance Liquid Chromatography (HPLC), which was previously known as high-pressure liquid chromatography, is the analytical chemistry’s technique that laboratories use to separate the components in a mixture and identify and quantify such components.

Mass Spectrometry (MS) is the analytical technique that laboratories use to measure the mass of every component contained in a sample. This technique ionizes the chemical species and then sorts the ions. Liquid Chromatography - Mass Spectrometry (LC-MS) and Liquid Chromatography - Tandem Mass Spectrometry (LC-MS2 or LC-MS-MS) is a method for testing that combines the capabilities of the Liquid Chromatography and the Mass Spectrometry to accurately identify substances contained in a sample. In the case of drugs, this technique is used by laboratories as a confirmation test on samples that are found to contain drug metabolites by an immunoassay test.

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