What form is used to document urine collection under DOT drug testing rules?


What form is used to document urine collection under DOT drug testing rules?


The Department of Transportation (DOT) has documented policies and procedures for drug testing and alcohol testing. 49 CFR part 40 covers the full details of these procedures.

Subpart D covers the collection sites, forms, equipment and supplies used in DOT urine collections. As highlighted in this section, the Federal Drug Testing Custody and Control Form (CCF) is used to document urine collection during DOT drug testing.

It is mandatory to use an official and up-to date (current) CCF form during the DOT drug testing process. The CCF form may not be modified except in the five instances outlined by DOT. Further to this:

Five copies of the form are used during the drug testing process, which go to designated people in each part of the collection process. There are a number of steps to completing the CCF form, as summarized below:

  1. Step one is completed by the collector or designated employer representative. This covers details such as the reasons for drug testing and which drug tests will be performed.
  2. Step two is completed by the collector to confirm specimen details such as temperature.
  3. Step three is completed by the collector and donor. Includes dating and sealing of specimen bottles.
  4. Step four is the chain of custody, which is started by the collector and completed by the testing lab.
  5. Step five is completed by the specimen donor to confirm that the specimen has not been adulterated and that correct policies and procedures have been followed
  6. Steps six (primary specimen) and seven (split specimen) are completed by the Medical Review Officer (MRO) to confirm the testing results.

Find out more about the differences between DOT and non-DOT drug testing in this article.

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John Hawes is the CCO and co-founder at SureHire Occupational Health Testing. John graduated in 2001 from the University of Alberta with a Bachelor of Science degree in physical therapy. As a former physical therapist, John uses his knowledge of physical therapy and interest in ergonomics and biomechanics to devise fit for work testing.

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